Benedict's Test for Glucose
Since this test detects any aldehydes and α-hydroxy ketones and glucose is an aldose whose open-chain forms an aldehyde group the test yields a positive result when glucose is present in the analyte. The ONPG test is considered to be a very sensitive test for lactose-fermentation. Benedict Test Principle Procedure Preparation Of Benedicts Reagent An Benedict Principles Test The Benedicts test identifies reducing sugars monosaccharides and some disaccharides which have free ketone or aldehyde functional groups. . Place two spatulas of the food sample into a test tube or 1 cm 3 if the sample is liquid. Monosaccharides can be classified by the number x of carbon atoms they contain. Some sugars such as glucose are called reducing sugars because they are capable of. During the next four to 10 minutes the solution should begin to change colors. It is also used for detecting glucose in urine as a presumptive test of diabetes. Ben...